The David H. Murdock Research Institute (DHMRI) and 10X Genomics are seeking scientists interested in working with them to develop new and expanded protocols for the company’s Chromium Controller and Solutions.
Kannapolis, NC - Although primarily supported for human samples, scientists in the Genomics Laboratory at the David H. Murdock Research Institute (DHMRI) are receiving requests to use the Chromium Controller and Solutions offered by 10X Genomics for everything from plants to marine animals.
Most recently, DHMRI Genomics staff have worked closely with scientists investigating flatworms and sea slugs to develop new protocols to extract and prepare fresh cells for sequencing with the Chromium Single Cell 3’ Solution. Overall, development of new protocols will leverage a wider variety of samples across the company’s platforms including advanced microfluidics and molecular barcoding for long-range whole genome sequencing, single-cell gene expressions and immune repertoire profiling.
“We recognize the value of 10X Genomics products and solutions,” stated Jorge Marques Signes, PhD, Group Leader of the DHMRI Genomics Laboratory. “We’re pleased to offer their technology in our Genomics Laboratory because of the versatility of the platform and our ability to help develop new applications.”
10X employs a Super-Poisson loading process to capture high molecular weight DNA or Single Cells into Gel Beads in Emulsion (GEMs) that are subsequently used for phasing and haplotyping of complex structural variants and for de novo assemblies. In single cell mode, the instrument can be used to generate single cell transcriptome data from 100-10,000 cells per well (or roughly 80,000 cells per chip), allowing for scalable gene expression profiling from mixed cell populations to achieve a better understanding of tumor microenvironments.
Simon Gregory, PhD, DHMRI Genomics Laboratory Director, noted that 10X recently added the Chromium Single Cell V(D)J Solution to profile and assemble full-length paired V(D)J transcripts on a cell-by-cell basis for hundreds to millions of lymphocytes, giving scientists greater insight into the immune system.
“This new 10X technology is useful for understanding markers predictive of a successful stem cell transplant and identifying invading lymphocytes and T cell profiles in tumors,” he commented. “As 10X continues to develop new genomics tools, we are looking forward to continuing our partnership and collaborating with more scientists to expand the potential application to as many types of organisms and sequencing challenges as possible.”
About the David H. Murdock Research Institute
The David H. Murdock Research Institute (DHMRI), located on the NC Research Campus in Kannapolis, NC, collaborates with scientists in academia, government and industry to provide a multidisciplinary, integrated, systems biology approach for solving scientific questions using analytical technologies such as genomics, metabolomics, proteomics, NMR and immune system profiling, particularly in the areas of human health, nutrition and agriculture. Learn more at www.dhmri.org.
About 10X Genomics
10x Genomics is changing the definition of sequencing by providing an innovative genomics platform that dramatically upgrades the capabilities of existing sequencing technologies. This is achieved through a combination of new microfluidic science, chemistry and bioinformatics. By implementing GemCode™ Technology within the Chromium™ System, researchers can now, for the first time, find new structural variants, haplotypes and other valuable genomic information with comprehensive workflows for Single Cell, V(D)J, Genome, Exome and de novo Assembly applications that incorporate their pre-existing sequencing technologies. Learn more at www.10xGenomics.com.