Next Generation Sequencing

With the Illumina HiSeq2500 sequencers, the DHMRI has the capability to run high throughput next generation sequencing for whole genomes, RNA-seq or specialized applications such as methylation sequencing, amplicon sequencing or exome capture. Illumina sequencing can be performed using a single read or paired end run. Read sizes include 50bp, 100bp, or 125bp in high output mode, and up to 250bp in rapid run mode. The Genomics laboratory can assist with all the phases of an experiment, from DNA or RNA extraction to library preparation, and sequencing.

The DHMRI Genomics laboratory performs a variety of library preparations for next generation sequencing to fit the needs of our clients. We have the ability to handle the more common applications for genome sequencing or RNA-seq, or more complex custom application such as exome capture or amplicon sequencing. Please see below for our list of applications. If you don’t see an application or service you are interested in, please contact us to discuss your project in more detail.

DNA Services    

  • Whole Genome    
  • Exome Capture     
  • Targeted Amplicon
  • Methylation
    • TrueMethyl Seq 
  • 10X Chromium Genome
  • Mate-Paired

RNA Services

The 10X Genomics Chromium has both DNA and RNA applications. The Chromium can be used to generate long-read genomic sequencing information from Illumina short read sequencing and can facilitate genome structure and haplotyping. This instrument can also be used to profile transcriptomes in a single cell format, allowing for profiling of gene expression from individual cells in a population.

The DHMRI is also a preferred provider for the Cambridge Epigenetix TrueMethyl Seq technology. This technology was developed to facilitate single-base resolution sequencing of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) and provide a unique understanding of the epigenetics of a sample.

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Genomics

The David H. Murdock Research Institute (DHMRI) Genomics Laboratory features a range of services and platforms that assist our collaborators with all phases of their projects from DNA or RNA extraction to library preparation and next generation sequencing. Our Genomics Laboratory also provides microarray and quantitative PCR platforms. Our scientists’ expertise allows them to develop novel methods, assays and protocols that give our collaborators custom applications and technologies.  All of the work conducted in the Genomics Laboratory is supported by state-of-the art computational and bioinformatics resources that help our collaborators get the most from their genetic data.

Collaborators also benefit from the availability of complementary services provided by DHMRI laboratories that specialize in metabolomics, proteomics, NMR, microscopy and targeted biochemistry.

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