Sample Preparation

Our Genomics Laboratory is equipped to extract nucleic acids from a variety of sample types, such as blood, cell cultures, tissue, swabs and fecal material, and perform the necessary quality controls. We use commercially available kits, and we can work with our collaborators to develop a protocol to suit their organism or tissue of interest. The Genomics Laboratory has the capacity to run large scale extractions using automation and also smaller scale pilot studies with reduced sample sizes. DNA and RNA can be extracted from fresh, frozen or preserved material. DNA quantitation is performed using a picogreen assay kit, and RNA is quantitated using a ribogreen assay kit. RNA quality is also visualized using the Agilent Bioanalyzer or the Agilent Tape Station. Additional quality measures can be completed upon request.

Genomics

The David H. Murdock Research Institute (DHMRI) Genomics Laboratory features a range of services and platforms that assist our collaborators with all phases of their projects from DNA or RNA extraction to library preparation and next generation sequencing. Our Genomics Laboratory also provides microarray and quantitative PCR platforms. Our scientists’ expertise allows them to develop novel methods, assays and protocols that give our collaborators custom applications and technologies.  All of the work conducted in the Genomics Laboratory is supported by state-of-the art computational and bioinformatics resources that help our collaborators get the most from their genetic data.

Collaborators also benefit from the availability of complementary services provided by DHMRI laboratories that specialize in metabolomics, proteomics, NMR, microscopy and targeted biochemistry.

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