Protein Structural Determination

Our scientists employ a variety of multiple high-field NMR instruments including a 700 and 950 MHz NMR to collect 2D and 3D multiple resonance experiments.  Edits and analysis of chemical shift assignments are processed using NMRPipe and NMRView. 

We use software packages such as CYANA and AMBER with NOESY NMR data to perform energy minimization and 3D structural refinement for total 3D structural determination.  NOESY or basic HSQC experiments are also used to determine protein-protein or protein-ligand binding experiments.  We apply relaxation experiments or user written pulse programs to highlight the dynamics of proteins.

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