Mass Spectrometry Identifies New Chemokine Heterodimers

Working with Irina Nesmelova, PhD, assistant professor in UNC Charlotte’s Department of Physics and Optical Sciences, scientist at the David H. Murdock Research Institute (DHMR) analyzed 11 chemokines using a ThermoOrbitrap ™ mass spectrometer with a nanoelectrospray ionization source. The method determined which chemokines most preferentially form heterodimers among an assortment of platelet-derived chemokines.

The preliminary data gained through the use of DHMRI’s mass spectrometry contributed to a National Institutes of Health grant application to further study CXCL4 and CXCL12, screen additional chemokines and conduct NMR experiments to build a structural model, which will assist in the design of a peptide to regulate the activity of these chemokines and breast cancer cell migration. These results were also published in the journal Biochimica et Biophysica Acta (BBA) in the paper “The heterodimerization of platelet-derived chemokines.”

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